The nucleus accumbens (Acb), a major sector of the ventral striatum, is considered to be an integral part of the striatal complex. The Acb has been shown to be composed of two subdivisions, core and shell, which are distinguishable in several aspects, suggesting that these two subdivisions play different functional roles. The aim of this study was to identify pathways of the efferents of the shell of the Acb to influence the outflow of the core of the Acb. Potential disynaptic projections of the shell to the core of the Acb were investigated in chloral hydrate-anesthetized male Sprague-Dawley rats. Following ipsilateral injections of biotinylated dextran amine (BDA) into the shell of the Acb and cholera toxin B subunit (CT-B) into the core, strong overlapping distributions of BDA-labeled terminals and CT-B-labeled neuronal cell somata were found in the medial part of the ventral tegmental area, medial part of the lateral hypothalamic area, and dorsolateral part of the basolateral amygdaloid nucleus. The significance of multiple sites of relay between the efferents of the shell and the afferents of the core of the Acb at different levels of the neuraxis may be related to the functional specificity of each relay site.