Abstract
The alpha-synuclein gene, which encodes a brain presynaptic nerve terminal protein of unknown function, is linked to familial early-onset Parkinson's disease (PD). The finding that alpha-synuclein forms the major fibrillary component of Lewy bodies in brains of PD patients suggests that the two point mutations in alpha-synuclein (Ala(53)Thr, Ala(30)Pro) may promote the aggregation of alpha-synuclein into filaments. To address the role of alpha-synuclein in neurodegenerative diseases, we performed a yeast two-hybrid screen of a rat adult brain cDNA library using rat alpha-synuclein 2 (alphaSYN2). Here we report that alphaSYN2 interacts specifically with Tat binding protein 1, a subunit of the 700-kDa proteasome activator (PA700), the regulatory complex of the 26S proteasome and of the modulator complex, which enhances PA700 activation of the proteasome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Animals
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Brain / cytology
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Brain / metabolism
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Cell Line
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Clone Cells
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DNA-Binding Proteins / metabolism*
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Dopamine / metabolism
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Humans
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Kidney / cytology
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Kidney / metabolism
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Lewy Bodies / metabolism
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Macromolecular Substances
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Neurons / cytology
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Neurons / metabolism
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Parkinsonian Disorders / metabolism
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Peptide Hydrolases / metabolism*
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Precipitin Tests
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Presynaptic Terminals / metabolism
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Proteasome Endopeptidase Complex*
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Proteins / metabolism
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Rats
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Saccharomyces / genetics
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Synucleins
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Transfection
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Two-Hybrid System Techniques
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Tyrosine 3-Monooxygenase / metabolism
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alpha-Synuclein
Substances
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DNA-Binding Proteins
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Macromolecular Substances
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Nerve Tissue Proteins
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PA700 proteasome activator
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Proteins
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SNCA protein, human
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Snca protein, rat
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Synucleins
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alpha-Synuclein
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Tyrosine 3-Monooxygenase
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Peptide Hydrolases
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Proteasome Endopeptidase Complex
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ATP dependent 26S protease
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ATPases Associated with Diverse Cellular Activities
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PSMC3 protein, human
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Dopamine