HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin

V S Chopra; M Metzler; D M Rasper; A E Engqvist-Goldstein; R Singaraja; L Gan; K M Fichter; K McCutcheon; D Drubin; D W Nicholson; M R Hayden

doi:10.1007/s003350010195

HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin

Mamm Genome. 2000 Nov;11(11):1006-15. doi: 10.1007/s003350010195.

Authors

V S Chopra¹, M Metzler, D M Rasper, A E Engqvist-Goldstein, R Singaraja, L Gan, K M Fichter, K McCutcheon, D Drubin, D W Nicholson, M R Hayden

Affiliation

¹ Department of Medical Genetics, and Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, Canada.

PMID: 11063258
DOI: 10.1007/s003350010195

Abstract

Huntingtin-interacting protein I (HIP1) is a membrane-associated protein that interacts with huntingtin, the protein altered in Huntington disease. HIP1 shows homology to Sla2p, a protein essential for the assembly and function of the cytoskeleton and endocytosis in Saccharomyces cerevisiae. We have determined that the HIP1 gene comprises 32 exons spanning approximately 215 kb of genomic DNA and gives rise to two alternate splice forms termed HIP1-1 and HIP1-2. Additionally, we have identified a novel protein termed HIP12 with significant sequence and biochemical similarities to HIP1 and high sequence similarity to Sla2p. HIP12 differs from HIP1 in its pattern of expression both at the mRNA and protein level. However, HIP1 and HIP12 are both found within the brain and show a similar subcellular distribution pattern. In contrast to HIP1, which is toxic in cell culture, HIP12 does not confer toxicity in the same assay systems. Interestingly, HIP12 does not interact with huntingtin but can interact with HIP1. suggesting a potential interaction in vivo that may influence the function of each respective protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alternative Splicing
Amino Acid Sequence
Base Sequence
Brain / metabolism
Caenorhabditis elegans Proteins*
Carrier Proteins / genetics*
Carrier Proteins / metabolism
Caspase 3
Caspases / metabolism
Cell Line
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / metabolism
DNA-Binding Proteins*
Fungal Proteins / genetics
Helminth Proteins / genetics
Humans
Huntingtin Protein
Kidney / cytology
Kidney / embryology
Kidney / metabolism
Molecular Sequence Data
Multigene Family
Nerve Tissue Proteins / metabolism*
Neurons / metabolism
Nuclear Proteins / metabolism*
Organ Specificity
Saccharomyces cerevisiae Proteins*
Sequence Homology, Amino Acid
Stem Cells / metabolism
Two-Hybrid System Techniques

Substances

Caenorhabditis elegans Proteins
Carrier Proteins
Cytoskeletal Proteins
DNA-Binding Proteins
Fungal Proteins
HIP1 protein, human
HTT protein, human
Helminth Proteins
Huntingtin Protein
Nerve Tissue Proteins
Nuclear Proteins
SLA2 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
hipr-1 protein, C elegans
CASP3 protein, human
Caspase 3
Caspases

Associated data

GENBANK/T08283
GENBANK/U79734