Abstract
In cultured hippocampal neurons, one axon and several dendrites differentiate from a common immature process. Here we found that CRMP-2/TOAD-64/Ulip2/DRP-2 (refs. 2-4) level was higher in growing axons of cultured hippocampal neurons, that overexpression of CRMP-2 in the cells led to the formation of supernumerary axons and that expression of truncated CRMP-2 mutants suppressed the formation of primary axon in a dominant-negative manner. Thus, CRMP-2 seems to be critical in axon induction in hippocampal neurons, thereby establishing and maintaining neuronal polarity.
MeSH terms
-
Animals
-
Cell Differentiation / genetics*
-
Cell Size / genetics*
-
Cells, Cultured / cytology
-
Cells, Cultured / metabolism*
-
Dendrites / metabolism
-
Dendrites / ultrastructure
-
GAP-43 Protein / metabolism
-
Growth Cones / metabolism*
-
Growth Cones / ultrastructure
-
Hippocampus / cytology
-
Hippocampus / embryology*
-
Hippocampus / metabolism
-
Immunohistochemistry
-
Intercellular Signaling Peptides and Proteins
-
Microtubule-Associated Proteins / metabolism
-
Mutation / physiology
-
Nerve Tissue Proteins / deficiency
-
Nerve Tissue Proteins / genetics*
-
Synapsins / metabolism
-
Synaptophysin / metabolism
-
Transfection
-
tau Proteins / metabolism
Substances
-
GAP-43 Protein
-
Intercellular Signaling Peptides and Proteins
-
Microtubule-Associated Proteins
-
Nerve Tissue Proteins
-
Synapsins
-
Synaptophysin
-
collapsin response mediator protein-2
-
tau Proteins