Abstract
The mammalian BAF complex regulates gene expression by modifying chromatin structure. In this report, we identify 80 genes activated and 2 genes repressed by the BAF complex in SW-13 cells. We find that prior binding of NFI/CTF to the NFI/CTF binding site in CSF1 promoter is required for the recruitment of the BAF complex and the BAF-dependent activation of the promoter. Furthermore, the activation of the CSF1 promoter requires Z-DNA-forming sequences that are converted to Z-DNA structure upon activation by the BAF complex. The BAF complex facilitates Z-DNA formation in a nucleosomal template in vitro. We propose a model in which the BAF complex promotes Z-DNA formation which, in turn, stabilizes the open chromatin structure at the CSF1 promoter.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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CCAAT-Enhancer-Binding Proteins / metabolism
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Chromatin / chemistry
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Chromatin / genetics
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Chromatin / metabolism
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DNA Helicases
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DNA, Superhelical / chemistry
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DNA, Superhelical / genetics
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DNA, Superhelical / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism*
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Gene Expression Profiling
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Gene Expression Regulation*
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Gene Silencing
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Humans
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Macromolecular Substances
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Macrophage Colony-Stimulating Factor / genetics*
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Models, Genetic
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Molecular Sequence Data
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NFI Transcription Factors
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Nucleic Acid Conformation
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Oligonucleotide Array Sequence Analysis
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Plasmids / chemistry
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Plasmids / genetics
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Plasmids / metabolism
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Promoter Regions, Genetic / genetics*
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Protein Binding
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Protein Subunits
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Response Elements / genetics
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Templates, Genetic
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcriptional Activation
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Tumor Cells, Cultured
Substances
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CCAAT-Enhancer-Binding Proteins
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CTF-1 transcription factor
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Chromatin
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DNA, Superhelical
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DNA-Binding Proteins
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Macromolecular Substances
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NFI Transcription Factors
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Nuclear Proteins
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Protein Subunits
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RNA, Messenger
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SMARCA2 protein, human
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Transcription Factors
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Macrophage Colony-Stimulating Factor
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SMARCA4 protein, human
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DNA Helicases