Regulation of CSF1 promoter by the SWI/SNF-like BAF complex

Cell. 2001 Aug 10;106(3):309-18. doi: 10.1016/s0092-8674(01)00446-9.

Abstract

The mammalian BAF complex regulates gene expression by modifying chromatin structure. In this report, we identify 80 genes activated and 2 genes repressed by the BAF complex in SW-13 cells. We find that prior binding of NFI/CTF to the NFI/CTF binding site in CSF1 promoter is required for the recruitment of the BAF complex and the BAF-dependent activation of the promoter. Furthermore, the activation of the CSF1 promoter requires Z-DNA-forming sequences that are converted to Z-DNA structure upon activation by the BAF complex. The BAF complex facilitates Z-DNA formation in a nucleosomal template in vitro. We propose a model in which the BAF complex promotes Z-DNA formation which, in turn, stabilizes the open chromatin structure at the CSF1 promoter.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins / metabolism
  • Chromatin / chemistry
  • Chromatin / genetics
  • Chromatin / metabolism
  • DNA Helicases
  • DNA, Superhelical / chemistry
  • DNA, Superhelical / genetics
  • DNA, Superhelical / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Gene Silencing
  • Humans
  • Macromolecular Substances
  • Macrophage Colony-Stimulating Factor / genetics*
  • Models, Genetic
  • Molecular Sequence Data
  • NFI Transcription Factors
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Nucleic Acid Conformation
  • Oligonucleotide Array Sequence Analysis
  • Plasmids / chemistry
  • Plasmids / genetics
  • Plasmids / metabolism
  • Promoter Regions, Genetic / genetics*
  • Protein Binding
  • Protein Subunits
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Response Elements / genetics
  • Templates, Genetic
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation
  • Tumor Cells, Cultured

Substances

  • CCAAT-Enhancer-Binding Proteins
  • CTF-1 transcription factor
  • Chromatin
  • DNA, Superhelical
  • DNA-Binding Proteins
  • Macromolecular Substances
  • NFI Transcription Factors
  • Nuclear Proteins
  • Protein Subunits
  • RNA, Messenger
  • SMARCA2 protein, human
  • Transcription Factors
  • Macrophage Colony-Stimulating Factor
  • SMARCA4 protein, human
  • DNA Helicases