Abstract
We screened for genes whose expression is significantly up- or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor-5 (FGF-5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF-5 in axotomized nerve. Axon-Schwann cell interactions decreased the steady-state level of FGF-5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF-5, which is a secreted, neuronotrophic member of the FGF family.
Copyright 2001 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Axons / physiology*
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Axotomy
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Cell Communication / physiology
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Cells, Cultured
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Colforsin / pharmacology
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Cyclic AMP / metabolism
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Fibroblast Growth Factor 5
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Fibroblast Growth Factors / genetics*
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Gene Expression / drug effects
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Gene Expression / physiology
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Nerve Regeneration / physiology*
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RNA, Messenger / analysis
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Rats
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Rats, Inbred Lew
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Schwann Cells / cytology
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Schwann Cells / physiology*
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Sciatic Nerve / cytology
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Sciatic Nerve / physiology*
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Wallerian Degeneration / physiopathology
Substances
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Fgf5 protein, rat
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RNA, Messenger
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Fibroblast Growth Factor 5
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Colforsin
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Fibroblast Growth Factors
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Cyclic AMP