Abstract
Transport of synaptic components is a regulated process. Loss-of-function mutations in the C. elegans unc-16 gene result in the mislocalization of synaptic vesicle and glutamate receptor markers. unc-16 encodes a homolog of mouse JSAP1/JIP3 and Drosophila Sunday Driver. Like JSAP1/JIP3, UNC-16 physically interacts with JNK and JNK kinases. Deletion mutations in Caenorhabditis elegans JNK and JNK kinases result in similar mislocalization of synaptic vesicle markers and enhance weak unc-16 mutant phenotypes. unc-116 kinesin heavy chain mutants also mislocalize synaptic vesicle markers, as well as a functional UNC-16::GFP. Intriguingly, unc-16 mutations partially suppress the vesicle retention defect in unc-104 KIF1A kinesin mutants. Our results suggest that UNC-16 may regulate the localization of vesicular cargo by integrating JNK signaling and kinesin-1 transport.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Animals
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Biological Transport, Active / genetics
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans / physiology*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Caenorhabditis elegans Proteins / physiology*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cell Line
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Drosophila
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Gene Deletion
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Green Fluorescent Proteins
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Humans
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JNK Mitogen-Activated Protein Kinases
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mice
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Mitogen-Activated Protein Kinases / genetics
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Mitogen-Activated Protein Kinases / metabolism*
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Molecular Sequence Data
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Mutation / physiology
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neurons / metabolism
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Proto-Oncogene Proteins c-jun / genetics
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Proto-Oncogene Proteins c-jun / metabolism*
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Rabbits
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Receptors, Glutamate / genetics
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Receptors, Glutamate / metabolism
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Signal Transduction / genetics
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Signal Transduction / physiology*
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Synaptic Vesicles / genetics
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Synaptic Vesicles / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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Caenorhabditis elegans Proteins
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Carrier Proteins
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Luminescent Proteins
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MAPK8IP3 protein, human
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Mapk8ip3 protein, mouse
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Nerve Tissue Proteins
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Proto-Oncogene Proteins c-jun
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Receptors, Glutamate
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unc-16 protein, C elegans
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Green Fluorescent Proteins
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases