Abstract
We have analyzed the expression and localization of bovine transient receptor potential-C1 (bTRPC1) in bovine aortic endothelial cells, and its possible involvement in the store-independent calcium influx induced by basic fibroblast growth factor (bFGF). RT-PCR experiments confirmed the existence of two btrpc1 mRNA isoforms; conversely, the btrpc3 gene was not transcribed. Anti-TRPC1 antibody revealed the presence of the protein in the membrane-rich compartment only. Application of anti-TRPC1 during the response to bFGF caused a partial but significant reduction of calcium entry. This is the first evidence of TRP channel involvement in a non-capacitative calcium influx induced by a biologically relevant agonist such as the angiogenic factor bFGF in native endothelial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Animals
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Antibodies / pharmacology
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Antibody Specificity
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Calcium / metabolism
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Calcium Channels / genetics
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Calcium Channels / metabolism*
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Cattle
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Cell Compartmentation / physiology
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Cell Line
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Cell Membrane / metabolism
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism*
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Fibroblast Growth Factor 2 / pharmacology
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Immunohistochemistry
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Ion Channels / genetics
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Ion Transport / drug effects
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Membrane Potentials / drug effects
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Patch-Clamp Techniques
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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TRPC Cation Channels
Substances
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Antibodies
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Calcium Channels
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Ion Channels
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RNA, Messenger
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TRPC Cation Channels
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TRPC3 cation channel
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transient receptor potential cation channel, subfamily C, member 1
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Fibroblast Growth Factor 2
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Calcium