Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I real-time RT-PCR

Anal Biochem. 2002 Apr 1;303(1):95-8. doi: 10.1006/abio.2001.5564.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles
  • DNA Primers
  • DNA, Complementary / analysis*
  • DNA, Complementary / metabolism
  • Deoxyribonucleases / metabolism
  • Diamines
  • Dimerization
  • Fluorescent Dyes / chemistry
  • Gene Expression Profiling
  • Organic Chemicals*
  • Quinolines
  • RNA / analysis
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction*

Substances

  • Benzothiazoles
  • DNA Primers
  • DNA, Complementary
  • Diamines
  • Fluorescent Dyes
  • Organic Chemicals
  • Quinolines
  • SYBR Green I
  • RNA
  • Deoxyribonucleases