Abstract
The role of an inositol 1,4,5-trisphosphate (IP3)-mediated transduction cascade in the response of taste receptor cells of the fleshfly Boettcherisca peregrina was investigated by using the following reagents: neomycin (an inhibitor of IP3 production), U73122 (an inhibitor of phospholipase C), adenophostin A (an agonist of the IP3-gated channel), IP3, ruthenium red (a blocker of the IP3-gated channel), and 2-aminoethoxydiphenylborate (2-APB; an antagonist of the IP3-gated channel). For introduction into the receptor cell, the reagents were mixed with a detergent, deoxycholate (DOC). After treatment with neomycin + DOC or U73122 + DOC, the response of the sugar receptor cell to sugars was depressed compared with responses after treatment with DOC alone. During the treatment of adenophostin A + DOC, the response of the sugar receptor cell was elicited. After treatment with IP3 + DOC, the response of the sugar receptor cell to sugars and to amino acids was apparently enhanced. When taste stimuli were administered in the presence of ruthenium red or 2-APB, the response of the sugar receptor cell to glucose were inhibited. The expression of genes for substances involved in the IP3 transduction cascade, such as G protein alpha subunit (dGqalpha), phospholipase C (norpA), and IP3 receptor (itpr), were examined in the taste receptor cell of the fruitfly Drosophila melanogaster by using the pox-neuro70 mutant (poxn70), which lacks taste receptor cells. The expressed levels of dGqalpha and itpr in the tarsus of poxn70 mutant flies were reduced compared with those of wild-type flies. These results suggest that the IP3 transduction cascade is involved in the response of the sugar receptor cell of the fly.
Copyright 2002 Wiley Periodicals, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine / analogs & derivatives*
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Adenosine / pharmacology
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Animals
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Animals, Genetically Modified
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Calcium Channel Agonists / pharmacology
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Calcium Channels / genetics
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Calcium Channels / metabolism*
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Deoxycholic Acid / pharmacology
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Detergents / pharmacology
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Diptera
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Drosophila Proteins*
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Drosophila melanogaster
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Estrenes / pharmacology
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GTP-Binding Protein alpha Subunits, Gq-G11
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GTP-Binding Proteins / metabolism
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Gene Expression / physiology
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Heterotrimeric GTP-Binding Proteins / genetics
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Indicators and Reagents / pharmacology
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Inositol 1,4,5-Trisphosphate / metabolism*
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Inositol 1,4,5-Trisphosphate Receptors
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Neomycin / pharmacology
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Nerve Tissue Proteins / genetics
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Paired Box Transcription Factors
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Phosphatidylinositol Diacylglycerol-Lyase
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Phosphodiesterase Inhibitors / pharmacology
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Phospholipase C beta
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Protein Synthesis Inhibitors / pharmacology
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Pyrrolidinones / pharmacology
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Ruthenium Red / pharmacology
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Signal Transduction / physiology*
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Taste / drug effects
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Taste / physiology*
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Transcription Factors / genetics
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Type C Phospholipases / genetics
Substances
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Calcium Channel Agonists
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Calcium Channels
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Detergents
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Drosophila Proteins
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Estrenes
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Indicators and Reagents
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Inositol 1,4,5-Trisphosphate Receptors
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Nerve Tissue Proteins
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Paired Box Transcription Factors
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Phosphodiesterase Inhibitors
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Poxn protein, Drosophila
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Protein Synthesis Inhibitors
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Pyrrolidinones
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Receptors, Cytoplasmic and Nuclear
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Transcription Factors
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Deoxycholic Acid
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Ruthenium Red
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1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
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adenophostin A
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Inositol 1,4,5-Trisphosphate
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Type C Phospholipases
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NorpA protein, Drosophila
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Phospholipase C beta
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GTP-Binding Proteins
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GTP-Binding Protein alpha Subunits, Gq-G11
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Heterotrimeric GTP-Binding Proteins
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Phosphatidylinositol Diacylglycerol-Lyase
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Neomycin
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Adenosine