Isolation and characterization of Golgi apparatus-specific GODZ with the DHHC zinc finger domain

Takeshi Uemura; Hisashi Mori; Masayoshi Mishina

doi:10.1016/s0006-291x(02)00900-2

Isolation and characterization of Golgi apparatus-specific GODZ with the DHHC zinc finger domain

Biochem Biophys Res Commun. 2002 Aug 16;296(2):492-6. doi: 10.1016/s0006-291x(02)00900-2.

Authors

Takeshi Uemura¹, Hisashi Mori, Masayoshi Mishina

Affiliation

¹ Department of Molecular Neurobiology and Pharmacology, Graduate School of Medicine, University of Tokyo, and SORST, Japan Science and Technology Corporation, Hongo 7-3-1, Bunkyo-ku, Tokyo, Japan.

PMID: 12163046
DOI: 10.1016/s0006-291x(02)00900-2

Abstract

We identified a novel Golgi apparatus-specific protein with the DHHC zinc finger domain and four putative transmembrane regions, designated as GODZ. The amino acid sequences were highly conserved among mouse and human GODZs and homologous proteins in human, mouse, rat, Drosophila melanogaster, and Caenorhabditis elegans, implying a functional significance of the GODZ protein family. Overexpression of mouse GODZ in COS7 cells suppressed the sorting of the glutamate receptor GluRalpha1 from the Golgi apparatus. These results suggest that GODZ plays a role in the membrane protein trafficking.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
COS Cells
Gene Library
Golgi Apparatus / chemistry
Golgi Apparatus / metabolism*
Humans
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / isolation & purification*
Membrane Proteins / metabolism*
Mice
Molecular Sequence Data
Phylogeny
Receptors, AMPA / genetics
Receptors, AMPA / metabolism
Sequence Alignment
Tissue Distribution
Two-Hybrid System Techniques
Zinc Fingers*

Substances

GODZ protein, mouse
Membrane Proteins
Receptors, AMPA
glutamate receptor ionotropic, AMPA 1

Associated data

GENBANK/AB088044