Abstract
Chicken prepro-orexin cDNA has been cloned, sequenced and characterized. The predicted amino acid sequence of chicken prepro-orexin cDNA revealed that orexin-A and -B are highly conserved among vertebrate species. In situ hybridization and immunohistochemistry localized orexin-positive cell bodies in the periventricular hypothalamic nucleus extending into the lateral hypothalamic area. Comparisons of orexin gene expression in the brains of 24-h-fasted and ad libitum-fed chickens were made using semi-quantitative RT-PCR. No significant differences in orexin mRNA expression were observed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chickens
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Cloning, Molecular
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DNA, Complementary / biosynthesis
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DNA, Complementary / chemistry
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Evolution, Molecular
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Hypothalamus / chemistry
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Hypothalamus / metabolism*
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Immunohistochemistry
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In Situ Hybridization
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Neuropeptides / biosynthesis
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Neuropeptides / chemistry
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Neuropeptides / genetics*
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Orexins
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Prosencephalon / metabolism
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Protein Precursors / biosynthesis
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Protein Precursors / chemistry
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Protein Precursors / genetics*
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RNA / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
Substances
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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Neuropeptides
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Orexins
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Protein Precursors
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RNA