Abstract
This study analyzes how the antigen specificity, the subtype, and the activation state of T cells modulate their recently discovered neuroprotective potential. We assessed the prevention from neuronal damage in organotypic entorhinal-hippocampal slice cultures after co-culture with Th1 and Th2 cells either specific for myelin basic protein (MBP) or ovalbumin (OVA). We found that MBP-specific Th2 cells were the most effective in preventing central nervous system (CNS) tissue from secondary injury. This neuroprotective T cell effect appears to be mediated by soluble factors. After stimulation with phorbol myristate acetate and ionomycin, all T cells were most effective in preventing neuronal death. Our data show that the T cell subtype and activation state are important features in determining the neuroprotective potential of these cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Brain / cytology
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Brain / immunology*
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Brain / physiopathology
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Brain Injuries / immunology*
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Brain Injuries / physiopathology
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Cell Survival / drug effects
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Cell Survival / immunology
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Chemotaxis, Leukocyte / drug effects
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Chemotaxis, Leukocyte / immunology*
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Contact Inhibition / immunology
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Cytokines / immunology
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Cytokines / metabolism
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Epitopes / immunology*
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Mice
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Mice, Inbred BALB C
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Myelin Basic Protein / immunology
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Myelin Basic Protein / pharmacology
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Nerve Degeneration / immunology*
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Nerve Degeneration / physiopathology
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Neurons / immunology
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Ovalbumin / immunology
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Ovalbumin / pharmacology
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Tetradecanoylphorbol Acetate / pharmacology
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Th1 Cells / cytology
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Th1 Cells / drug effects
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Th1 Cells / immunology*
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Th2 Cells / cytology
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Th2 Cells / drug effects
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Th2 Cells / immunology*
Substances
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Cytokines
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Epitopes
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Myelin Basic Protein
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Ovalbumin
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Tetradecanoylphorbol Acetate