The CD26-related dipeptidyl aminopeptidase-like protein DPPX is a critical component of neuronal A-type K+ channels

Marcela S Nadal; Andrés Ozaita; Yimy Amarillo; Eleazar Vega-Saenz de Miera; Yuliang Ma; Wenjun Mo; Ethan M Goldberg; Yoshio Misumi; Yukio Ikehara; Thomas A Neubert; Bernardo Rudy

doi:10.1016/s0896-6273(02)01185-6

The CD26-related dipeptidyl aminopeptidase-like protein DPPX is a critical component of neuronal A-type K+ channels

Neuron. 2003 Feb 6;37(3):449-61. doi: 10.1016/s0896-6273(02)01185-6.

Authors

Marcela S Nadal¹, Andrés Ozaita, Yimy Amarillo, Eleazar Vega-Saenz de Miera, Yuliang Ma, Wenjun Mo, Ethan M Goldberg, Yoshio Misumi, Yukio Ikehara, Thomas A Neubert, Bernardo Rudy

Affiliation

¹ Department of Physiology and Neuroscience and Department of Biochemistry, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.

PMID: 12575952
DOI: 10.1016/s0896-6273(02)01185-6

Abstract

Subthreshold-activating somatodendritic A-type potassium channels have fundamental roles in neuronal signaling and plasticity which depend on their unique cellular localization, voltage dependence, and kinetic properties. Some of the components of A-type K(+) channels have been identified; however, these do not reproduce the properties of the native channels, indicating that key molecular factors have yet to be unveiled. We purified A-type K(+) channel complexes from rat brain membranes and found that DPPX, a protein of unknown function that is structurally related to the dipeptidyl aminopeptidase and cell adhesion protein CD26, is a novel component of A-type K(+) channels. DPPX associates with the channels' pore-forming subunits, facilitates their trafficking and membrane targeting, reconstitutes the properties of the native channels in heterologous expression systems, and is coexpressed with the pore-forming subunits in the somatodendritic compartment of CNS neurons.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Cell Adhesion / physiology
Cerebellum / cytology
Dendrites / enzymology
Dipeptidyl Peptidase 4 / genetics
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Extracellular Matrix / metabolism
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / metabolism
Molecular Sequence Data
Nerve Tissue Proteins / chemistry
Nerve Tissue Proteins / genetics*
Nerve Tissue Proteins / metabolism*
Neurons / enzymology*
Neurons / ultrastructure
Oocytes / physiology
Potassium Channels / chemistry
Potassium Channels / genetics*
Potassium Channels / metabolism*
Potassium Channels, Voltage-Gated*
Precipitin Tests
Protein Transport / physiology
Rats
Rats, Sprague-Dawley
Shal Potassium Channels
Xenopus

Substances

Membrane Proteins
Nerve Tissue Proteins
Potassium Channels
Potassium Channels, Voltage-Gated
Shal Potassium Channels
DPP6 protein, rat
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Dipeptidyl Peptidase 4

Grants and funding

1R21NS44184/NS/NINDS NIH HHS/United States