SOX10 maintains multipotency and inhibits neuronal differentiation of neural crest stem cells

Jaesang Kim; Liching Lo; Emma Dormand; David J Anderson

doi:10.1016/s0896-6273(03)00163-6

SOX10 maintains multipotency and inhibits neuronal differentiation of neural crest stem cells

Neuron. 2003 Apr 10;38(1):17-31. doi: 10.1016/s0896-6273(03)00163-6.

Authors

Jaesang Kim¹, Liching Lo, Emma Dormand, David J Anderson

Affiliation

¹ Howard Hughes Medical Institute, Division of Biology 216-76, California Institute of Technology, Pasadena, California 91125, USA.

PMID: 12691661
DOI: 10.1016/s0896-6273(03)00163-6

Abstract

The mechanisms that establish and maintain the multipotency of stem cells are poorly understood. In neural crest stem cells (NCSCs), the HMG-box factor SOX10 preserves not only glial, but surprisingly, also neuronal potential from extinction by lineage commitment signals. The latter function is reflected in the requirement of SOX10 in vivo for induction of MASH1 and PHOX2B, two neurogenic transcription factors. Simultaneously, SOX10 inhibits or delays overt neuronal differentiation, both in vitro and in vivo. However, this activity requires a higher Sox10 gene dosage than does the maintenance of neurogenic potential. The opponent functions of SOX10 to maintain neural lineage potentials, while simultaneously serving to inhibit or delay neuronal differentiation, suggest that it functions in stem or progenitor cell maintenance, in addition to its established role in peripheral gliogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / drug effects
Cell Differentiation / physiology
Cell Division / drug effects
Cell Division / physiology
Cell Lineage / physiology
Cells, Cultured
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Down-Regulation / physiology
Gene Expression Regulation, Developmental
High Mobility Group Proteins / genetics
High Mobility Group Proteins / metabolism*
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism
Multipotent Stem Cells / cytology*
Multipotent Stem Cells / physiology
Nerve Tissue Proteins
Neural Crest / cytology*
Neuroglia / cytology*
Neurons / cytology*
Rats
SOXE Transcription Factors
Transcription Factors / genetics
Transcription Factors / metabolism
Transcription, Genetic / physiology
Transforming Growth Factor beta / pharmacology

Substances

DNA-Binding Proteins
High Mobility Group Proteins
Homeodomain Proteins
Nerve Tissue Proteins
Phox2a protein, rat
SOXE Transcription Factors
Sox10 protein, rat
Transcription Factors
Transforming Growth Factor beta