Abstract
In primary cultured striatal neurons we found that (+-)-trans-1-amino-cyclopentyl-1,3-dicarboxylate (trans-ACPD) could inhibit forskolin-induced cAMP formation in a dose-dependent manner (EC50 156 +/- 38 microM, n = 5, maximal inhibition 37.8 +/- 1.2, n = 37). The trans-ACPD-induced inhibition was totally abolished in neurons preincubated with Bordetella pertussis toxin (1 microgram/ml), demonstrating the involvement of a G-protein. This is the first report in intact neurons of a glutamate metabotropic receptor negatively coupled to cAMP formation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Colforsin / pharmacology
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Corpus Striatum / cytology
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Corpus Striatum / drug effects
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Corpus Striatum / metabolism*
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Cyclic AMP / metabolism*
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Cycloleucine / analogs & derivatives*
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Cycloleucine / pharmacology
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Dose-Response Relationship, Drug
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GTP-Binding Proteins / metabolism*
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Pertussis Toxin
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Virulence Factors, Bordetella / pharmacology
Substances
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Virulence Factors, Bordetella
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Cycloleucine
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1-amino-1,3-dicarboxycyclopentane
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Colforsin
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Cyclic AMP
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Pertussis Toxin
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GTP-Binding Proteins