Combinatorial RNA splicing alters the surface charge on the NMDA receptor

V Anantharam; R G Panchal; A Wilson; V V Kolchine; S N Treistman; H Bayley

doi:10.1016/0014-5793(92)80648-z

Combinatorial RNA splicing alters the surface charge on the NMDA receptor

FEBS Lett. 1992 Jun 22;305(1):27-30. doi: 10.1016/0014-5793(92)80648-z.

Authors

V Anantharam¹, R G Panchal, A Wilson, V V Kolchine, S N Treistman, H Bayley

Affiliation

¹ Department of Pharmacology, University of Massachusetts Medical Center, Worcester 01655.

PMID: 1386026
DOI: 10.1016/0014-5793(92)80648-z

Abstract

Transcripts encoding four NMDA receptor subunits, generated from the NMDAR1 gene by alternative RNA splicing, have been demonstrated in adult rat brain. RNA transcripts derived from cDNAs encoding each form direct the formation of functional NMDA receptors in Xenopus oocytes. The two amino acid cassettes of 21 and 37 amino acids found in the splice variants increase the positive extracellular surface charge on the subunits and may thereby modulate the functional properties of the receptor.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
DNA
Electrochemistry
Molecular Sequence Data
Polymerase Chain Reaction
RNA Splicing*
Rats
Receptors, N-Methyl-D-Aspartate / chemistry
Receptors, N-Methyl-D-Aspartate / genetics*

Substances

Receptors, N-Methyl-D-Aspartate
DNA

Associated data

GENBANK/S86392
GENBANK/S90294
GENBANK/S90296
GENBANK/S90298
GENBANK/X65227
GENBANK/X65503
GENBANK/X65504
GENBANK/X66534
GENBANK/Z11695
GENBANK/Z11696