sup-9, sup-10, and unc-93 may encode components of a two-pore K+ channel that coordinates muscle contraction in Caenorhabditis elegans

J Neurosci. 2003 Oct 8;23(27):9133-45. doi: 10.1523/JNEUROSCI.23-27-09133.2003.

Abstract

Genetic studies of sup-9, unc-93, and sup-10 strongly suggest that these genes encode components of a multi-subunit protein complex that coordinates muscle contraction in Caenorhabditis elegans. We cloned sup-9 and sup-10 and found that they encode a two-pore K+ channel and a novel transmembrane protein, respectively. We also found that UNC-93 and SUP-10 colocalize with SUP-9 within muscle cells, and that UNC-93 is a member of a novel multigene family that is conserved among C. elegans, Drosophila, and humans. Our results indicate that SUP-9 and perhaps other two-pore K+ channels function as multiprotein complexes, and that UNC-93 and SUP-10 likely define new classes of ion channel regulatory proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / drug effects
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Cloning, Molecular
  • Exons
  • GABA Agonists / pharmacology
  • GTPase-Activating Proteins / genetics
  • Green Fluorescent Proteins
  • Introns
  • Luminescent Proteins / biosynthesis
  • Luminescent Proteins / genetics
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Motor Activity / drug effects
  • Motor Activity / genetics
  • Motor Activity / physiology
  • Muscimol / pharmacology
  • Muscle Contraction / drug effects
  • Muscle Contraction / physiology*
  • Muscle Proteins / metabolism*
  • Nerve Tissue Proteins / genetics
  • Organ Specificity / genetics
  • Phenotype
  • Potassium Channels / genetics
  • Potassium Channels / metabolism*
  • Potassium Channels, Tandem Pore Domain*
  • Protein Subunits / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Sequence Homology, Amino Acid

Substances

  • BEM2 protein, S cerevisiae
  • Caenorhabditis elegans Proteins
  • GABA Agonists
  • GTPase-Activating Proteins
  • Luminescent Proteins
  • Membrane Proteins
  • Muscle Proteins
  • Nerve Tissue Proteins
  • Potassium Channels
  • Potassium Channels, Tandem Pore Domain
  • Protein Subunits
  • Saccharomyces cerevisiae Proteins
  • Sup-10 protein, C elegans
  • Sup-9 protein, C elegans
  • unc-93 protein, C elegans
  • Green Fluorescent Proteins
  • potassium channel subfamily K member 3
  • Muscimol