Abstract
The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific siRNA that selective silences LRP expression in human aortic smooth muscle cells. As a consequence, LRP-mediated ligand degradation was significantly reduced. More important, we found that platelet-derived growth factor-dependent cell migration was inhibited in cells transfected with LRP siRNA. These results demonstrate an important role of LRP in smooth muscle cell migration.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aorta / cytology
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Base Sequence
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Binding Sites
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Cell Line
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Cell Movement / physiology*
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Gene Silencing
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Humans
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LDL-Receptor Related Protein-Associated Protein / pharmacology
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LDL-Receptor Related Proteins / physiology*
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Muscle, Smooth, Vascular / cytology*
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Plasminogen Activator Inhibitor 1 / chemistry
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Plasminogen Activator Inhibitor 1 / metabolism
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Platelet-Derived Growth Factor / antagonists & inhibitors
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Platelet-Derived Growth Factor / metabolism
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RNA, Small Interfering / genetics
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RNA, Small Interfering / pharmacology
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Recombinant Proteins / metabolism
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Transfection
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Urokinase-Type Plasminogen Activator / chemistry
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Urokinase-Type Plasminogen Activator / metabolism
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alpha-Macroglobulins / metabolism
Substances
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LDL-Receptor Related Protein-Associated Protein
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LDL-Receptor Related Proteins
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Plasminogen Activator Inhibitor 1
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Platelet-Derived Growth Factor
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RNA, Small Interfering
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Recombinant Proteins
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alpha-Macroglobulins
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Urokinase-Type Plasminogen Activator