Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome

Biotechniques. 2003 Dec;35(6):1170-4, 1176, 1178 passim. doi: 10.2144/03356st02.

Abstract

The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description for visually preclassifying mice to be genotyped.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, U.S. Gov't, P.H.S.
  • Validation Study

MeSH terms

  • Algorithms*
  • Animals
  • Disease Models, Animal
  • Down Syndrome / diagnosis*
  • Down Syndrome / genetics*
  • Gene Expression Profiling / methods*
  • Genetic Testing / methods*
  • Genotype
  • Mice
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity