A neuronal isoform of CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia

Cell. 2003 Dec 26;115(7):893-904. doi: 10.1016/s0092-8674(03)01021-3.

Abstract

Synapse-specific facilitation requires rapamycin-dependent local protein synthesis at the activated synapse. In Aplysia, rapamycin-dependent local protein synthesis serves two functions: (1) it provides a component of the mark at the activated synapse and thereby confers synapse specificity and (2) it stabilizes the synaptic growth associated with long-term facilitation. Here we report that a neuron-specific isoform of cytoplasmic polyadenylation element binding protein (CPEB) regulates this synaptic protein synthesis in an activity-dependent manner. Aplysia CPEB protein is upregulated locally at activated synapses, and it is needed not for the initiation but for the stable maintenance of long-term facilitation. We suggest that Aplysia CPEB is one of the stabilizing components of the synaptic mark.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Animals
  • Aplysia
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • Drosophila Proteins / genetics
  • Drosophila Proteins / isolation & purification
  • Drosophila Proteins / metabolism
  • Long-Term Potentiation / physiology*
  • Molecular Sequence Data
  • Nerve Tissue Proteins / biosynthesis*
  • Poly A / metabolism
  • Protein Biosynthesis / physiology
  • Protein Isoforms / metabolism
  • RNA Stability / physiology
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Serotonin / metabolism
  • Serotonin / pharmacology
  • Synapses / drug effects
  • Synapses / metabolism*
  • Synaptic Transmission / drug effects
  • Synaptic Transmission / physiology*
  • Tacrolimus Binding Proteins / genetics
  • Tacrolimus Binding Proteins / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*
  • Up-Regulation / drug effects
  • Up-Regulation / physiology
  • Xenopus Proteins / genetics
  • Xenopus Proteins / isolation & purification
  • Xenopus Proteins / metabolism*
  • mRNA Cleavage and Polyadenylation Factors

Substances

  • Actins
  • Cpeb1 protein, Xenopus
  • DNA, Complementary
  • Drosophila Proteins
  • Nerve Tissue Proteins
  • Protein Isoforms
  • RNA, Messenger
  • Transcription Factors
  • Xenopus Proteins
  • mRNA Cleavage and Polyadenylation Factors
  • Poly A
  • Serotonin
  • Cyclic AMP-Dependent Protein Kinases
  • Tacrolimus Binding Proteins