Abstract
Synapse-specific facilitation requires rapamycin-dependent local protein synthesis at the activated synapse. In Aplysia, rapamycin-dependent local protein synthesis serves two functions: (1) it provides a component of the mark at the activated synapse and thereby confers synapse specificity and (2) it stabilizes the synaptic growth associated with long-term facilitation. Here we report that a neuron-specific isoform of cytoplasmic polyadenylation element binding protein (CPEB) regulates this synaptic protein synthesis in an activity-dependent manner. Aplysia CPEB protein is upregulated locally at activated synapses, and it is needed not for the initiation but for the stable maintenance of long-term facilitation. We suggest that Aplysia CPEB is one of the stabilizing components of the synaptic mark.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / genetics
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Animals
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Aplysia
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Cyclic AMP-Dependent Protein Kinases / metabolism
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Drosophila Proteins / genetics
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Drosophila Proteins / isolation & purification
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Drosophila Proteins / metabolism
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Long-Term Potentiation / physiology*
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Molecular Sequence Data
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Nerve Tissue Proteins / biosynthesis*
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Poly A / metabolism
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Protein Biosynthesis / physiology
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Protein Isoforms / metabolism
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RNA Stability / physiology
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Serotonin / metabolism
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Serotonin / pharmacology
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Synapses / drug effects
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Synapses / metabolism*
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Synaptic Transmission / drug effects
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Synaptic Transmission / physiology*
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Tacrolimus Binding Proteins / genetics
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Tacrolimus Binding Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism*
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Up-Regulation / drug effects
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Up-Regulation / physiology
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Xenopus Proteins / genetics
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Xenopus Proteins / isolation & purification
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Xenopus Proteins / metabolism*
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mRNA Cleavage and Polyadenylation Factors
Substances
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Actins
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Cpeb1 protein, Xenopus
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DNA, Complementary
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Drosophila Proteins
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Nerve Tissue Proteins
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Protein Isoforms
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RNA, Messenger
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Transcription Factors
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Xenopus Proteins
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mRNA Cleavage and Polyadenylation Factors
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Poly A
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Serotonin
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Cyclic AMP-Dependent Protein Kinases
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Tacrolimus Binding Proteins