Abstract
We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cloning, Molecular
-
Embryo, Nonmammalian / cytology
-
Embryo, Nonmammalian / metabolism
-
Gene Expression Regulation, Developmental / physiology*
-
Genes, Reporter / genetics
-
Genetic Vectors
-
Green Fluorescent Proteins
-
Luminescent Proteins / metabolism
-
Microinjections
-
Motor Neurons / cytology
-
Motor Neurons / metabolism*
-
Peripheral Nervous System / cytology
-
Peripheral Nervous System / metabolism*
-
Purkinje Cells / cytology
-
Purkinje Cells / metabolism*
-
Zebrafish / anatomy & histology
-
Zebrafish / genetics*
-
Zebrafish / metabolism
Substances
-
Luminescent Proteins
-
Green Fluorescent Proteins