Abstract
Quantitative polymerase chain reaction (Q-PCR) is now considered the "technique of choice" for validating gene expression changes identified with ribonucleic acid-based expression profiling technologies (especially micro- and macroarray techniques). The identification of altered gene expression profiles with microarrays is best viewed as the first step in the determination of potential disease-associated genes; however, the false-positive rate can be high, particularly with small sample sets and in view of the typically small differences observed in brain expression studies. Quantitative PCR is a rapid and highly sensitive technique for accurate quantification of microarray results; however, careful consideration of experimental design, quality of primer/probe design, internal standards, and normalization procedures are pivotal, particularly when the work involves postmortem tissue.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Apolipoprotein L1
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Apolipoproteins / genetics
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Apolipoproteins / metabolism
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Benzothiazoles
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Brain Chemistry / genetics*
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Diamines
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Gene Expression
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Humans
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Lipoproteins, HDL / genetics
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Lipoproteins, HDL / metabolism
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Myelin Basic Protein / genetics
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Myelin Basic Protein / metabolism
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Myelin Proteolipid Protein / genetics
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Myelin Proteolipid Protein / metabolism
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Oligonucleotide Array Sequence Analysis / methods*
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Oligonucleotide Array Sequence Analysis / standards
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Organic Chemicals / metabolism
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Polymerase Chain Reaction / methods*
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Postmortem Changes
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Quinolines
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RNA, Messenger / metabolism
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Reproducibility of Results
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Schizophrenia / genetics
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Schizophrenia / metabolism
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Sensitivity and Specificity
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Statistics, Nonparametric
Substances
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APOL1 protein, human
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Apolipoprotein L1
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Apolipoproteins
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Benzothiazoles
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Diamines
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Lipoproteins, HDL
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Myelin Basic Protein
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Myelin Proteolipid Protein
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Organic Chemicals
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Quinolines
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RNA, Messenger
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SYBR Green I