Plasmid-based short-hairpin RNA interference in the chicken embryo

Genesis. 2004 Jun;39(2):73-8. doi: 10.1002/gene.20028.

Abstract

The chicken neural tube serves as an ideal model for new techniques to alter gene expression. We show that short-hairpin RNA delivered by a plasmid vector can effectively knock down expression of both exogenous and endogenous genes in the chicken neural tube. We also assay the effects of short-hairpin RNA on the interferon response and find no difference between electroporation of a control plasmid and a short-hairpin RNA plasmid.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chick Embryo / metabolism*
  • Electroporation
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Interferons / pharmacology
  • LIM-Homeodomain Proteins
  • Mice
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Nervous System / embryology*
  • Plasmids / genetics*
  • RNA Interference*
  • RNA Polymerase III / metabolism
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism
  • Transcription Factors

Substances

  • Homeodomain Proteins
  • LIM-Homeodomain Proteins
  • Nerve Tissue Proteins
  • RNA, Small Interfering
  • Transcription Factors
  • insulin gene enhancer binding protein Isl-1
  • Green Fluorescent Proteins
  • Interferons
  • RNA Polymerase III