Abstract
The chicken neural tube serves as an ideal model for new techniques to alter gene expression. We show that short-hairpin RNA delivered by a plasmid vector can effectively knock down expression of both exogenous and endogenous genes in the chicken neural tube. We also assay the effects of short-hairpin RNA on the interferon response and find no difference between electroporation of a control plasmid and a short-hairpin RNA plasmid.
Copyright 2004 Wiley-Liss, Inc.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chick Embryo / metabolism*
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Electroporation
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Gene Expression Regulation, Developmental*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Interferons / pharmacology
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LIM-Homeodomain Proteins
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Mice
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Nervous System / embryology*
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Plasmids / genetics*
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RNA Interference*
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RNA Polymerase III / metabolism
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RNA, Small Interfering / genetics*
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RNA, Small Interfering / metabolism
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Transcription Factors
Substances
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Homeodomain Proteins
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LIM-Homeodomain Proteins
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Nerve Tissue Proteins
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RNA, Small Interfering
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Transcription Factors
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insulin gene enhancer binding protein Isl-1
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Green Fluorescent Proteins
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Interferons
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RNA Polymerase III