AF1q, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron-like cells

Hsingchi J Lin; Kara M Shaffer; Zairen Sun; Gilbert Jay; Wei-wu He; Wu Ma

doi:10.1016/j.molbrainres.2004.07.022

AF1q, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron-like cells

Brain Res Mol Brain Res. 2004 Nov 24;131(1-2):126-30. doi: 10.1016/j.molbrainres.2004.07.022.

Authors

Hsingchi J Lin¹, Kara M Shaffer, Zairen Sun, Gilbert Jay, Wei-wu He, Wu Ma

Affiliation

¹ Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA. jlin@cbmse.nrl.navy.mil

PMID: 15530661
DOI: 10.1016/j.molbrainres.2004.07.022

Abstract

Three cell groups, neural stem/progenitor cells (NS/PCs) dissociated from the embryonic day 11 (E11) rodent cerebral cortex, expanded NS/PC cultures, and cultured neurons from E15, were used to conduct a genomic study with differential display (DD). The mouse Af1q, homologue of human AF1q, was found to be significantly up-regulated during the neuronal production from NS/PCs. The ectopic expression of human AF1q triggered the expression of the neuronal marker TuJ1 in non-neuronal human embryonic kidney (HEK) cells.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Biomarkers
Blood Proteins / genetics*
Cell Differentiation / physiology
Cells, Cultured
Gene Expression Regulation, Developmental
Humans
Kidney / cytology*
Mice
Neoplasm Proteins / genetics*
Neurons / cytology*
Neurons / physiology
Proto-Oncogene Proteins
Stem Cells / cytology*
Stem Cells / physiology
Transfection
Tubulin / genetics

Substances

Biomarkers
Blood Proteins
MLLT11 protein, human
Neoplasm Proteins
Proto-Oncogene Proteins
Tubulin
beta3 tubulin, mouse