Gene expression profiling in whole cerebral cortices of phencyclidine- or methamphetamine-treated rats

Yuta Ouchi; Yasuhiko Kubota; Atsuo Kuramasu; Takehiko Watanabe; Chihiro Ito

doi:10.1016/j.molbrainres.2005.07.011

Gene expression profiling in whole cerebral cortices of phencyclidine- or methamphetamine-treated rats

Brain Res Mol Brain Res. 2005 Oct 31;140(1-2):142-9. doi: 10.1016/j.molbrainres.2005.07.011. Epub 2005 Aug 24.

Authors

Yuta Ouchi¹, Yasuhiko Kubota, Atsuo Kuramasu, Takehiko Watanabe, Chihiro Ito

Affiliation

¹ Department of Psychiatry, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.

PMID: 16122833
DOI: 10.1016/j.molbrainres.2005.07.011

Abstract

Both phencyclidine (PCP) and methamphetamine (MAP) can cause schizophrenia-like symptoms. To identify the molecules relating to the drug-induced psychotic state, we used serial analysis of gene expression in rodent cerebral cortices isolated 1 h after intraperitoneal injection of saline, PCP (10 mg/kg), or MAP (4 mg/kg). We analyzed a total of 150,000 tags and found significantly up- or down-regulated genes. The number of MAP-, PCP-, and MAP and PCP-reactive tags were 229, 215, and 41, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cerebral Cortex / drug effects
Cerebral Cortex / physiology*
DNA Damage
DNA Primers
Gene Expression Profiling / methods*
Gene Expression Regulation / drug effects*
Injections, Intraperitoneal
Methamphetamine / administration & dosage
Methamphetamine / pharmacology*
Nerve Tissue Proteins / genetics
Phencyclidine / administration & dosage
Phencyclidine / pharmacology*
Rats

Substances

DNA Primers
Nerve Tissue Proteins
Methamphetamine
Phencyclidine