Abstract
The B2 molecule is a 220 kd neuronal cell surface protein of Xenopus, recognized by monoclonal antibody B2 (MAb B2). Immunohistochemistry using MAb B2 revealed that the B2 molecule was expressed in both the inner and outer plexiform layers within the neural retina. During development of the neural retina, the B2 molecule first appeared at stages 35/36 in the newly formed plexiform layers. When embryonic eyes were cultured in the presence of anti-B2 antiserum (Fab fragments), the formation of the retinal plexiform layers was impeded. These data suggest that the cell surface molecule B2 plays a role in the development of retinal plexiform layers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal
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Cell Adhesion Molecules / analysis
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / physiology*
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Cell Differentiation / physiology
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Cell Membrane / chemistry
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Cell Membrane / ultrastructure
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Cells, Cultured
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Immunoglobulin Fab Fragments
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Immunohistochemistry
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Microscopy, Immunoelectron
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / immunology
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Nerve Tissue Proteins / physiology*
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Neurons / chemistry
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Neurons / cytology*
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Neurons / ultrastructure
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Organ Culture Techniques
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Retina / chemistry
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Retina / cytology
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Retina / embryology*
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Xenopus
Substances
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Antibodies, Monoclonal
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Cell Adhesion Molecules
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Immunoglobulin Fab Fragments
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Nerve Tissue Proteins
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plexin