Abstract
New Zealand white rabbits were immunized with a lipopolysaccharide (LPS) extracted from a Campylobacter jejuni HS:19 strain isolated from a GBS patient expressing GM1 and GD1a-like epitopes, Freund's adjuvant (group I) and Freund's adjuvant plus keyhole lympet hemocyanin (KLH) (group II). Both groups showed high titers of anti-LPS and anti-GM1 and lower titers of anti-GD1b and anti-GD1a antibodies. Weakness and axonal degeneration in sciatic nerves was detected in 1/11 of group I and 6/7 of group II. This model replicates, at least in part, the pathogenetic process hypothesized in the human axonal GBS with antiganglioside antibodies post C. jejuni infection and indicates that KLH plays an additional role in neuropathy induction.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Bacterial / biosynthesis
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Blotting, Western / methods
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Campylobacter jejuni / chemistry*
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Campylobacter jejuni / immunology
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Disease Models, Animal
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Electrophoresis, Polyacrylamide Gel / methods
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Enzyme-Linked Immunosorbent Assay / methods
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Epitopes
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G(M1) Ganglioside / immunology
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Gangliosides / immunology
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Gene Expression / immunology
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Guillain-Barre Syndrome / immunology
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Guillain-Barre Syndrome / microbiology*
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Humans
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Immunization
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Lipopolysaccharides / immunology*
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Male
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Polyradiculoneuropathy / etiology*
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Polyradiculoneuropathy / immunology
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Polyradiculoneuropathy / pathology
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Rabbits
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Time Factors
Substances
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Antibodies, Bacterial
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Epitopes
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Gangliosides
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Lipopolysaccharides
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ganglioside, GD1a
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G(M1) Ganglioside