Abstract
A neuronal antigen (HuD) recognized by the sera of patients with antibody-associated paraneoplastic encephalomyelitis has been isolated by screening a lambda cerebellar expression library. The recombinant antigen provides an unambiguous assay for this rare condition associated with small cell lung cancer. The recombinant antigen has been used to identify specific infiltrating lymphocytes in tumors and affected brain tissues of patients with antibody-associated paraneoplastic encephalomyelitis and sensory neuronopathy. HuD mRNA is uniquely expressed in brain tissue. The HuD protein shows a remarkable homology to the Drosophila proteins Elav and Sex-lethal and is likely to play a role in neuron-specific RNA processing.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Neoplasm / genetics*
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Antigens, Neoplasm / immunology
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Base Sequence
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Brain / immunology
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Carcinoma, Small Cell / immunology
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Cloning, Molecular
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Drosophila / genetics
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ELAV Proteins
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ELAV-Like Protein 4
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Encephalomyelitis / immunology*
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Gene Expression / physiology
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Humans
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Lung Neoplasms / immunology
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Lymphocytes / immunology
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / immunology
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Paraneoplastic Syndromes / immunology*
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RNA, Messenger / analysis
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RNA-Binding Proteins / genetics*
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RNA-Binding Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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Ribonucleoproteins / genetics
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Sequence Homology, Nucleic Acid
Substances
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Antigens, Neoplasm
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ELAV Proteins
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ELAV-Like Protein 4
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ELAVL4 protein, human
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Nerve Tissue Proteins
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RNA, Messenger
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Ribonucleoproteins
Associated data
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GENBANK/M62843
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GENBANK/M81934
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GENBANK/M82983
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GENBANK/M82984
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GENBANK/M82985
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GENBANK/M82986
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GENBANK/M82987
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GENBANK/M82988
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GENBANK/M82989
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GENBANK/M82990
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GENBANK/S60140