Separation of alpha 1, alpha 2 and alpha 3 subunits of the GABAA-benzodiazepine receptor complex by immunoaffinity chromatography

Brain Res. 1991 Nov 1;563(1-2):325-8. doi: 10.1016/0006-8993(91)91556-g.

Abstract

Polyclonal antibodies raised to synthetic amino acid sequences of the rat gamma-aminobutyric acid (GABAA) receptor alpha 1-, alpha 2- and alpha 3-subunits selectively recognized single proteins with apparent molecular weight 51 kDa (P51), 53 kDa (P53) and 59 kDa (P59), respectively, in GABAA receptor preparations affinity purified from the brains of 5-10-day-old rats. The antibodies were coupled to Affigel 10, and the resulting immunoaffinity columns were used to isolate these proteins from affinity purified GABAA receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Chromatography, Affinity
  • Molecular Sequence Data
  • Molecular Weight
  • Peptides / analysis
  • Rats
  • Receptors, GABA-A / chemistry*
  • Receptors, GABA-A / immunology

Substances

  • Peptides
  • Receptors, GABA-A