Abstract
We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / chemistry
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Action Potentials / drug effects
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Animals
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Brain Mapping / instrumentation
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Brain Mapping / methods*
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Calcium Signaling / drug effects
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Cytophotometry / instrumentation
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Cytophotometry / methods*
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Electrophysiology
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Excitatory Postsynaptic Potentials / drug effects
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Glutamates / metabolism
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Glutamine / pharmacology
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Indoles / chemistry
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Indoles / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Microscopy, Fluorescence, Multiphoton / instrumentation
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Microscopy, Fluorescence, Multiphoton / methods*
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Neocortex / cytology
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Neocortex / drug effects
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Neocortex / physiology
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Nerve Net / cytology
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Nerve Net / metabolism
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Nerve Net / physiology*
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Neurons / cytology
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Neurons / drug effects
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Neurons / physiology
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Reproducibility of Results
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Tetrodotoxin / pharmacology
Substances
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4-methoxy-7-nitroindolinyl-glutamate
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Acetates
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Glutamates
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Indoles
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mag-indo-1 AM
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Glutamine
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Indo-1 pentaacetoxymethyl ester
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Tetrodotoxin