Abstract
delta-catenin is a protein that binds to the classical cadherins and to synaptic scaffolding proteins in a manner which allows the protein to serve as a link between the adherens junction and the postsynaptic complex. Here we show the regulatory role of delta-catenin on synaptic transmission. delta-catenin increased the AMPA receptor-mediated EPSC, but had no significant effect on the NMDA receptor-mediated EPSC. The effect of delta-catenin on the AMPAR EPSC was mediated by its PDZ ligand. delta-catenin regulates the surface expression of GluR2 in the dendritic spines of neurons. Immunoprecipitation revealed that delta-catenin bound to GRIP-1. In COS7 cells, co-transfection of delta-catenin, GRIP and GluR2 showed that delta-catenin regulates the membrane localization of GRIP through its PDZ ligand and increased the surface expression of GluR2. This study directly shows that delta-catenin is essential for the trafficking and positioning GluR2 in the spine and thus further links delta-catenin to neuronal plasticity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Catenins
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / metabolism*
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Cells, Cultured
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Chlorocebus aethiops
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Delta Catenin
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Excitatory Postsynaptic Potentials / physiology
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Hippocampus / cytology
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Intracellular Signaling Peptides and Proteins
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neuronal Plasticity / physiology
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Neurons / cytology
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Neurons / metabolism
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Patch-Clamp Techniques
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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RNA Interference
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Rats
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Rats, Sprague-Dawley
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Receptors, AMPA / genetics
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Receptors, AMPA / metabolism*
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Receptors, N-Methyl-D-Aspartate / genetics
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Receptors, N-Methyl-D-Aspartate / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Synaptic Transmission / physiology*
Substances
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Carrier Proteins
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Catenins
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Cell Adhesion Molecules
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Grip1 protein, rat
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Intracellular Signaling Peptides and Proteins
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Nerve Tissue Proteins
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Phosphoproteins
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Receptors, AMPA
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Receptors, N-Methyl-D-Aspartate
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Recombinant Fusion Proteins
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glutamate receptor ionotropic, AMPA 2
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Delta Catenin