Abstract
The processes underlying long-term memory formation in the neocortex are poorly understood. Using taste learning, we found learning-related induction of PSD-95 in the gustatory cortex, which was temporally restricted, coupled to the learning of a novel, but not familiar, taste and controlled by ERK. Using temporally and spatially restricted RNA interference knockdown of PSD-95 in vivo, we found that PSD-95 induction is necessary for learning novel tastes, but not for the recollection of familiar ones.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Analysis of Variance
-
Animals
-
Anisomycin / pharmacology
-
Avoidance Learning / physiology*
-
Behavior, Animal
-
Conditioning, Operant / drug effects
-
Conditioning, Operant / physiology
-
Disks Large Homolog 4 Protein
-
Excitatory Postsynaptic Potentials / drug effects
-
Excitatory Postsynaptic Potentials / physiology
-
Excitatory Postsynaptic Potentials / radiation effects
-
Extracellular Signal-Regulated MAP Kinases / metabolism*
-
Green Fluorescent Proteins / metabolism
-
Hippocampus / cytology
-
In Vitro Techniques
-
Intracellular Signaling Peptides and Proteins / genetics
-
Intracellular Signaling Peptides and Proteins / metabolism*
-
Male
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism*
-
Protein Synthesis Inhibitors / pharmacology
-
RNA Interference / physiology
-
Rats
-
Rats, Sprague-Dawley
-
Rats, Wistar
-
Somatosensory Cortex / drug effects
-
Somatosensory Cortex / physiology*
-
Taste / physiology*
-
Transfection
Substances
-
Disks Large Homolog 4 Protein
-
Dlg4 protein, rat
-
Intracellular Signaling Peptides and Proteins
-
Membrane Proteins
-
Protein Synthesis Inhibitors
-
enhanced green fluorescent protein
-
Green Fluorescent Proteins
-
Anisomycin
-
Extracellular Signal-Regulated MAP Kinases