Abstract
The olfactory epithelium (OE) is a site of massive adult neurogenesis where olfactory sensory neurons (OSNs) are continuously turned over. Tissue macrophages have been implicated in phagocytosis of degenerating cells but the molecular mechanisms that allow for their recruitment while maintaining a neurogenic microenvironment are poorly understood. This study reports that the neuroprotective chemokine CX3CL1 is expressed by OSNs and olfactory ensheathing cells. Monocyte-derived cells in the OE depend on CX3CL1-signalling for intraepithelial migration and apical dendrite expression. These observations are first to demonstrate phenotypic differences in appearance and distribution of monocyte-derived cells in nervous tissue due to CX3CR1 deficiency.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CX3C Chemokine Receptor 1
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Cell Count / methods
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Cell Movement / genetics
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Cell Movement / physiology*
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Dendrites / physiology*
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Female
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Green Fluorescent Proteins / genetics
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Transgenic
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Monocytes / physiology
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Neurogenesis / genetics*
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Olfactory Mucosa / cytology*
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Olfactory Mucosa / metabolism
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Olfactory Receptor Neurons / cytology
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Olfactory Receptor Neurons / physiology*
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RNA, Messenger / metabolism
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Rats
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Rats, Inbred F344
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Receptors, Chemokine / deficiency
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Receptors, Chemokine / physiology*
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Receptors, Cytokine / genetics
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Receptors, Cytokine / metabolism
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Receptors, HIV / genetics
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Receptors, HIV / metabolism
Substances
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CX3C Chemokine Receptor 1
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Cx3cr1 protein, mouse
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RNA, Messenger
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Receptors, Chemokine
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Receptors, Cytokine
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Receptors, HIV
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Green Fluorescent Proteins