Abstract
We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Biosensing Techniques / methods
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Brain / cytology
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Brain / physiology
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Green Fluorescent Proteins / analysis*
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Green Fluorescent Proteins / biosynthesis
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Green Fluorescent Proteins / genetics
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Herpesvirus 1, Suid / genetics
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Herpesvirus 1, Suid / metabolism*
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Luminescent Proteins / analysis*
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Luminescent Proteins / biosynthesis
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Luminescent Proteins / genetics
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Mice
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Neurons / physiology
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Neurons / virology
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Red Fluorescent Protein
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Synaptic Transmission / physiology*
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Time Factors
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Visual Pathways / physiology
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Visual Pathways / virology*
Substances
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Luminescent Proteins
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Green Fluorescent Proteins