Abstract
In generating a conditional transgenic murine model based on a tetracycline-regulated system, we obtained unexpected patterns of expression due to the transcriptional inactivity of the tet-responder promoter. Here we show strong cell-type-restricted expression that was variegated to an extent determined by the number of responder transgene copies integrated into the host genome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Gene Dosage / physiology
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Gene Expression Regulation / drug effects*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Mice
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Mice, Transgenic
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Models, Biological
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Olfactory Nerve / metabolism
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Organ Specificity / genetics
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Protein Synthesis Inhibitors / pharmacology
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Superoxide Dismutase / genetics
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Superoxide Dismutase / metabolism
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Superoxide Dismutase-1
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Tetracycline / pharmacology*
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Transgenes / drug effects*
Substances
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Protein Synthesis Inhibitors
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Sod1 protein, mouse
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Superoxide Dismutase
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Superoxide Dismutase-1
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Tetracycline