Abstract
A cDNA for a member of the G protein-coupled receptor family was isolated from Drosophila using a probe derived from a human beta 2-adrenergic receptor cDNA. This Drosophila receptor gene is localized at 99A10-B1 on the right arm of chromosome 3 and is preferentially expressed in Drosophila heads. The insect octopamine receptor has been permanently expressed in mammalian cells, where it mediates the attenuation of adenylate cyclase activity and exhibits a pharmacological profile consistent with an octopamine type 1 receptor. Sequence and pharmacological comparisons indicate that the octopamine receptor is unique but closely related to mammalian adrenergic receptors, perhaps as an evolutionary precursor.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism
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Cell Line
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Chromosome Mapping
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Cloning, Molecular / methods
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DNA / genetics
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DNA / isolation & purification
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Drosophila / genetics*
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GTP-Binding Proteins / genetics
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Gene Expression*
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Genes*
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Genomic Library
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Humans
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Molecular Sequence Data
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Octopamine / metabolism
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Oligonucleotide Probes
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Protein Conformation
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Receptors, Adrenergic / genetics*
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Receptors, Adrenergic, beta / genetics
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Receptors, Biogenic Amine*
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Restriction Mapping
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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Oligonucleotide Probes
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Receptors, Adrenergic
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Receptors, Adrenergic, beta
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Receptors, Biogenic Amine
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norsynephrine receptor
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Octopamine
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DNA
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GTP-Binding Proteins