The effects of adrenergic and excitatory amino acid antagonists on supraoptic nucleus (SON) neurosecretory cell responses to stimulation of the A1 noradrenaline (NA) cell group were examined in anaesthetized male rats. As in previous studies, delivery of cathodal pulses (100 microA, 1 ms pulses, 1 Hz) to the A1 region of the caudal ventrolateral medulla excited spontaneously active, antidromically identified neurosecretory cells, the majority of which were identified as arginine vasopressin (AVP) secreting on the basis of basal discharge patterns and responses to abrupt increases in arterial blood pressure. Administration of alpha- and beta-adrenoreceptor antagonists, by systemic or intracerebroventricular delivery of a bolus, or by direct pressure injection into the SON, did not alter neurosecretory cell responses to A1 stimulation, even when doses applied exceeded that required for blockade of excitations elicited by local application of NA. Application of the broad spectrum excitatory amino acid antagonist kynurenic acid (5-40 mM) blocked the excitatory effects of locally applied glutamate (100 microM) and transiently inhibited spontaneous activity, but failed to alter the excitatory effects of A1 region stimulation on SON cells. Identical effects were obtained with a selective kainate/quisqualate receptor antagonist. These data indicate that neurosecretory cell responses to activation of the A1 cell group are unaltered by antagonists of alpha- and beta-adrenoreceptors, or excitatory amino acid receptors.(ABSTRACT TRUNCATED AT 250 WORDS)