Abstract
Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson's disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated in the pathogenesis of Parkinson's disease. Developing an assay to understand the mechanisms of LRRK2 kinase activity is important for the development of pharmacologic and therapeutic applications. Here, we describe how to measure in vitro LRRK2 kinase activity and its inhibition.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Line, Transformed
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Enzyme Activation / drug effects
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Enzyme Activation / genetics
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Enzyme Assays / methods*
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Gene Expression Regulation
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HEK293 Cells
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Humans
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Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
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Myelin Basic Protein / metabolism
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Parkinson Disease / enzymology*
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Parkinson Disease / genetics
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Phosphorylation
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
Substances
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Myelin Basic Protein
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Recombinant Fusion Proteins
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LRRK2 protein, human
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Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
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Protein Serine-Threonine Kinases