Abstract
The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cell Differentiation / genetics
-
Chromatin Immunoprecipitation
-
DNA-Binding Proteins / metabolism
-
Embryonic Stem Cells / cytology*
-
Embryonic Stem Cells / metabolism*
-
Gene Expression Regulation, Developmental*
-
Genome-Wide Association Study
-
High-Throughput Nucleotide Sequencing
-
Mice
-
Motor Neurons / cytology
-
Motor Neurons / metabolism
-
Sequence Analysis, DNA
-
Transcription Factors / metabolism*
Substances
-
DNA-Binding Proteins
-
Transcription Factors