Adding sucrose to the low calcium bathing solution made with no added calcium but containing Mg2+ EGTA to increase the tonicity elevated the basal frequency of miniature end-plate potentials (MEPPs) in the frog. The hypertonic low calcium solution also increased the rate at which the MEPP frequency rose in response to tetanic stimulation and elevated the level of the maximum frequency, which approached an asymptote. Pretreatment with concanavalin A (con A) greatly reduced the elevation in the basal frequency produced by hypertonic solutions. However, tetanic stimulation gave the same results as in untreated preparations when the tonicity was increased. Pretreatment with colchicine before the con A treatment eliminated the blocking action of con A on the MEPP frequency when the preparation was exposed to hypertonic solutions. Tetanic stimulation produced increases in MEPP frequency similar to those observed in normal junctions immersed in hypertonic solutions. Caffeine elevated the basal level of the MEPP frequency. Tetanic stimulation in the caffeine solution caused the increase in the MEPP frequency; the higher the basal level rose, the higher the maximum level became. However, the rate at which the MEPP frequency rose remained unchanged. The present results indicate that hypertonicity increases not only the basal frequency of MEPPs but also the slope at which the MEPP frequency is elevated by tetanic stimulation, both mechanisms being different and that the rate and magnitude of the tetanic potentiation of MEPP frequency are not simply determined by the pre-tetanus frequency.