Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers on single pudendal motoneurons in Onuf's nucleus of the cat: a light microscope study combining the triple immunocytochemical staining technique with the retrograde HRP-tracing method

Brain Res. 1989 Mar 6;481(2):392-8. doi: 10.1016/0006-8993(89)90821-4.

Abstract

Convergence of serotonin (5-HT)-, enkephalin (ENK)-, and substance P (SP)-like immunoreactive (LI) afferent fibers on single pudendal motoneurons within Onuf's nucleus was demonstrated: pudendal motoneurons were retrogradely labeled with horseradish peroxidase applied to the pudendal nerve. Subsequently, 5-HT-LI fibers were stained by the immunoperoxidase method, and then ENK- and SP-LI fibers were stained by the double immunofluorescence method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anal Canal / innervation*
  • Animals
  • Cats
  • Enkephalins / metabolism*
  • Female
  • Horseradish Peroxidase
  • Immunohistochemistry
  • Male
  • Motor Neurons / metabolism*
  • Neurons, Afferent / metabolism*
  • Serotonin / metabolism*
  • Substance P / metabolism*
  • Urethra / innervation*

Substances

  • Enkephalins
  • Serotonin
  • Substance P
  • Horseradish Peroxidase