Abstract
Chronic infusion of nerve growth factor (NGF) into the forebrain of the adult rat produced increases in NGF receptor (NGF-R) mRNA hybridization, NGF-R immunoreactivity, choline acetyltransferase (ChAT) mRNA hybridization, and neuronal hypertrophy, when compared with vehicle infusion or noninfused rat brain. In situ hybridization showed NGF induction of NGF-R gene expression, documented by increases in the number of NGF-R mRNA-positive cells within the medial septum, diagonal band, and nucleus basalis magnocellularis. NGF also produced hypertrophy of ChAT mRNA-positive neurons. These results suggest that NGF produces cholinergic neuronal hypertrophy through induction of NGF-R gene expression within the basal forebrain.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Brain / drug effects*
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Brain / metabolism
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Brain / pathology
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Choline O-Acetyltransferase / genetics
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Choline O-Acetyltransferase / metabolism
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Cholinergic Fibers / drug effects
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Cholinergic Fibers / metabolism
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Cholinergic Fibers / pathology*
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Female
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Gene Expression / drug effects
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Hypertrophy / chemically induced
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Hypertrophy / pathology
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Immunohistochemistry
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Nerve Growth Factors / pharmacology*
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Neurons / drug effects
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Neurons / metabolism
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Neurons / pathology*
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Nucleic Acid Hybridization
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Inbred Strains
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Receptors, Cell Surface / drug effects
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Receptors, Cell Surface / genetics*
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Receptors, Nerve Growth Factor
Substances
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Nerve Growth Factors
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RNA, Messenger
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Receptors, Cell Surface
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Receptors, Nerve Growth Factor
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Choline O-Acetyltransferase