Reciprocal regulation of tachykinin- and vasoactive intestinal peptide-gene expression in rat sensory neurones following cut and crush injury

Brain Res. 1989 Feb 27;481(1):25-30. doi: 10.1016/0006-8993(89)90481-2.

Abstract

The relative abundance of preprotachykinin- (PPT), actin- and vasoactive intestinal peptide- (VIP) mRNA's was measured in L5 dorsal root ganglia of rats after resecting or crushing the sciatic nerve. PPT-mRNA levels fell to 40% of control values 3, 6 and 9 days following nerve resection. Crushing produced a lesser fall at 3 and 6 days with a partial recovery at 9 days. Following resection actin-mRNA levels transiently rose to twice control values and had returned to normal by day 9. VIP-mRNA was not detectable in control ganglia but increasing amounts of VIP-mRNA were present 3, 6 and 9 days after nerve injury. The results are discussed in terms of the control mechanisms operating.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Ganglia, Spinal / metabolism*
  • Ganglia, Spinal / physiology
  • Gene Expression Regulation*
  • Male
  • Nerve Crush
  • Nerve Regeneration*
  • Neurons, Afferent / metabolism*
  • Neurons, Afferent / physiology
  • Protein Precursors / metabolism*
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Tachykinins / metabolism*
  • Vasoactive Intestinal Peptide / metabolism*

Substances

  • Actins
  • Protein Precursors
  • RNA, Messenger
  • Tachykinins
  • preprotachykinin
  • Vasoactive Intestinal Peptide