Abstract
Although neuroblast generation is highly reproducible temporospatially, the underlying regulation is undefined. Employing a fully defined sympathetic neuroblast culture system, we previously found that insulin growth factors regulated the mitotic cycle. We now report that depolarizing stimuli, including elevated K+ and veratridine, also increased the proportion of mitotic neuroblasts in vitro. Moreover, Na+ channel blockade prevented effects of depolarization, but not that of insulin-like growth factor I, suggesting that these influences stimulate mitosis by different membrane transduction mechanisms.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Cycle / drug effects
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Cells, Cultured
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Embryo, Mammalian
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Ganglia, Sympathetic / cytology*
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Ganglia, Sympathetic / drug effects
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Ganglia, Sympathetic / physiology
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Insulin-Like Growth Factor I / pharmacology*
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Mitosis / drug effects*
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Potassium / pharmacology*
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Rats
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Rats, Inbred Strains
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Sodium / physiology
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Somatomedins / pharmacology*
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Tetrodotoxin / pharmacology
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Thymidine
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Veratridine / pharmacology*
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Veratrine / analogs & derivatives*
Substances
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Somatomedins
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Tetrodotoxin
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Insulin-Like Growth Factor I
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Veratridine
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Sodium
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Veratrine
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Potassium
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Thymidine