In vivo microdialysis was used to monitor bilaterally the release of dopamine and its metabolites 3,4-dihydroxyphenyl acetic acid (DOPAC) and homovanillic acid (HVA) in the striata of both anesthetized and awake, freely moving female rats. Under baseline conditions, an asymmetry in dopamine release was reciprocally related to an asymmetry in DOPAC. Baseline dopamine and DOPAC asymmetries were predictive of the preferred direction of amphetamine-induced rotation: the striatum having higher dopamine and lower DOPAC was contralateral to the preferred direction of rotation. Amphetamine (D-amphetamine sulfate, 1.25 mg/kg) enhanced dopamine release and decreased DOPAC and HVA; the increase in dopamine was greater in the ipsilateral striatum. Effects in anesthetized and awake rats were similar. Variations in rotation and in the dopamine asymmetry after amphetamine were correlated across time within individual awake rats.