Montage: a system for three-dimensional reconstruction by personal computer

J Neurosci Methods. 1987 Sep;21(1):55-69. doi: 10.1016/0165-0270(87)90102-6.

Abstract

This paper describes a simplified system for serial section three-dimensional (3-D) reconstruction. A set of 9 software programs runs on a standard personal computer and produces camera-ready illustrations suitable for publication. The user enters trace points on a digitizing tablet from sections that have been already aligned. A 3-D view of the reconstructed object is generated which can be displayed with hidden lines removed. Analysis of volume, surface area and autoradiographic grain density are performed automatically. A relational database query language allows display and analysis of a selected subset of the data. The system runs under the UNIX operating system which allows the programs to be easily transported to new hardware or modified for other purposes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Computer Graphics*
  • Computers*
  • Histological Techniques*
  • Microcomputers*
  • Software*