Glia-axon protein transfer was examined in the squid giant axon. Proteins synthesized by the glial sheath surrounding the axon were labeled with [3H]leucine. Raising the temperature of the incubation medium from 20 degrees C to 30 degrees C increased the synthesis of glial proteins that resembled heat-shock proteins. These proteins were among the group known to be transferred into the axon. Thus, glia provide the axon with proteins that may be involved in the reaction to trauma.