Afferent connections to the medial hypothalamic region in the rat were studied using horseradish peroxidase (HRP). HRP was injected iontophoretically by a parapharyngeal approach. After HRP injections into the ventromedial hypothalamic nucleus, labeled cells were found mainly in the medial and basolateral amygdaloid nuclei, subiculum, peripeduncular nucleus and the parabrachial area. Labeled cells following HRP injections into the dorsomedial hypothalamic nucleus were found mainly in the lateral septal nucleus, nucleus accumbens, bed nucleus of the stria terminalis, pontine central gray and the parabrachial area. HRP-labeled cells following the medial preoptic area injections were found mainly in the infralimbic cortex, lateral and medial septal nuclei, nucleus accumbens, diagonal band, bed nucleus of the stria terminalis, medial amygdaloid nucleus, subiculum, peripeduncular nucleus and the parabrachial area. The intrahypothalamic connections were also discussed.