The distribution of motoneurons in the lumbosacral spinal cord of the turtle Pseudemys scripta elegans was studied by using the technique of retrograde transport of horseradish peroxidase. A total of 19 different hindlimb muscles were injected with varying amounts of horseradish peroxidase. The resulting distribution of labeled motoneurons was studied in both longitudinal and transverse sections of spinal cord. Motoneurons innervating a particular hindlimb muscle are clustered in longitudinally arranged motorpools. Motorpools of different muscles can show considerable overlap in both the rostrocaudal and transverse planes. The distribution of the various motorpools demonstrates a somatotopic organization of motoneurons within the lumbar spinal cord. Motoneurons innervating more distally positioned muscles are generally found in the more caudal segments, while motoneurons supplying proximal muscles are distributed throughout almost the whole lumbosacral intumescence. Motoneurons innervating anterodorsally positioned muscles are found in the ventrolateral part of area IX in the ventral horn, while more dorsomedially positioned motoneurons innervate the posteroventral muscles. These features are consistent with observations in other tetrapods, although the somatotopic representation of motoneurons is more evident in higher vertebrates such as chicken and cat. The observed motorpool distribution is discussed in relation to the presumed ontogeny of the spinal cord and hindlimb muscles and also in relation to the functions of the investigated muscles.